Biography
Biography: Uma Nahar Saikia
Abstract
Title: Role of HPV 6/11 in Giant condylomas in Indian patients.
Uma Nahar Saikia1, Suvradeep Mitra1, Tirupti Rangta2, Mini P Singh2, and Dipankar De3.
Departments of Histopathology, Virology and Dermatology and Venereology and Leprology, Postgraduate Institute of medical Education and research, Chandigarh, India
Introduction: Genital warts are quite common in sexually active population with Human papilloma virus (HPV) as the causative agent. In western literature HPV6/11 as an etiological factor varies from 80 to 90% , however there is a paucity of literature in India about the type of virus causing condylomata .
Material and Methods: A total of 22 histologically confirmed cases of condylomata acuminata were included in the study over a period of 2 years(2014-2016). The majority (19/22; 86.3%) of the biopsy samples were from genital & perianal areas. Formalin Fixed Para-film (FFPE) embedded sections were used for HPV 6 &11 using 2-3, 20-30 micron sections. The targets used were L1 & E6 region of HPV 6 and 11 respectively.
Result: Clinically, 18 patients had giant condylomas and 4 had multiple lesions. There was male preponderance (95.4%) with mean age of 46.3 (18-84 years). Histologically marked acanthosis with papillomatosis was noted. There was moderately dense lymphoplasmacytic infiltrate in the upper dermis with vascular proliferation. Koilocytic change was seen in 10 (71.4%) cases which correlated with PCR positivity in 10 cases.. On PCR 14 cases (63.6%) were positive for HPV 6 or 11; HPV 6 alone was present in eight cases (36.3%) and HPV 11 in six cases (27.2%). No dual infection was present. The HPV 6/11 positivity correlated with higher degree of inflammation. Three cases with clinical diagnosis of syphilis, Ewing’s sarcoma and carcinoma breast repectively were negative for HPV 6 & HPV 11.
Conclusion: The PCR results confirmed the presence of HPV6/11 in 63.6% of condylomata acuminata cases. Five morphologically confirmed cases were negative for HPV 6 & 11, possibly due to late stage of infection with no significant inflammation or koilocytic change. Hence HPV vaccination to be used in prevention of giant condylomas caused by HPV 6 or 11.