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Senthamarai Thiyagarajan

Senthamarai Thiyagarajan

Meenakshi Medical College Hospital and Research Institute, India

Title: Detection of mecA gene by PCR among coagulase negative Staphylococci (CoNS) from various clinical samples in a tertiary care hospital

Biography

Biography: Senthamarai Thiyagarajan

Abstract

Coagulase Negative Staphylococci (CoNS) are now emerging as important pathogen and its resistance to antibiotics are worrisome. Methicillin resistance among CoNS causes an important therapeutic threat associated with increased morbidity and mortality. This study was aimed to isolate the coagulase negative Staphylococci from various clinical specimens, evaluate its antibioticsusceptibility pattern and to detect the prevalence of mecA gene among coagulase negative Staphylococci.All the clinical samples were collected with aseptic precautions and processed as per standard protocol. All the coagulase negative Staphylococcal isolates were subjected for antibiotic susceptibility testing as per CLSI guidelines. Screening of methicillin resistance was done using cefoxitin disc (30 μg) as per CLSI recommended disc diffusion method. Genotypic analysis for methicillin resistance (mecA gene) was done.